K17905

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Elements::Others::P

Original: K17905

Determination of introduced cDNA(s)

J023010C06 - Rice Full-length cDNAs - Original: J023010C06

full reading [+] show sequence

GGGCGGCCTC CTCCTCTCCT CCTCCCCTGC ACAGACCACT CGTTTCCTCC 
ACAAAGAGGG AGGGAACAAG GGAAGGGTGT CGCCCGCCCC CCACCCCGAT 
CTGCCTCCGC CGCTCCGCTC CTCCGCGCCT GCGAAATCTA CCAACGCTAA 
CTCAGCAAGA TGGGTGCCGG CGGCAGGATG ACGGAGAAGG AGCGGGAGGA 
GCAGCAGAAG CTGCTCGGCC GCGCCGGCAA TGGCGCGGCC GTGCAGCGGT 
CGCCGACGGA CAAGCCGCCG TTCACGCTGG GGCAGATCAA GAAGGCCATC 
CCGCCTCACT GCTTCCAGCG CTCGGTGATC AAGTCCTTCT CCTACGTGGT 
CCATGACCTC GTGATCGTCG CCGCGCTGCT CTACTTCGCG CTGGTCATGA 
TCCCCGTGCT GCCGAGCGGG ATGGAGTTCG CGGCATGGCC GCTCTACTGG 
ATCGCGCAGG GCTGCGTGCT CACCGGCGTG TGGGTCATCG CGCACGAGTG 
CGGCCACCAT GCCTTCTCCG ACTACTCGGT GCTCGACGAC ATCGTCGGCC 
TCGTGCTGCA CTCGTCGCTG CTCGTCCCCT ACTTCTCGTG GAAGTACAGC 
CACCGGCGCC ACCACTCCAA CACCGGGTCG CTGGAGCGCG ACGAGGTGTT 
CGTCCCGAAG CAGAAGTCGG CGATGGCGTG GTACACCCCG TACGTGTACC 
ACAACCCGAT CGGCCGGCTG GTGCACATCT TCGTGCAGCT CACCCTCGGG 
TGGCCGCTGT AACTGGCGTT CAACGTGTCC GGCCGCCCGT ACCCGCGCTT 
CGCGTGCCAC TTCGACCCCT ACGGCCCGAT CTACAACGAC CGGGAGCGCG 
TCCAGATCTT CATCTCCGAC GTCGGCGTCG TGTCCGCGGG GCTCGCCCTG 
TTCAAGCTGT CGTCGGCGTT CGGGTTCTGG TGGGTGGTGC GCGTCTACGG 
CGTGCCGCTG CTGATCGTGA ACGCGTGGCT GGTGCTCATC ACCTACCTGC 
AGCACACCCA CCCGGCGCTG CCGCACTACG ACTCGAGCGA GTGGGACTGG 
CTCCGCGGCG CGCTGGCCAC CGTGGACCGC GACTACGGCA TCCTCAACAA 
GGTGTTCCAC AACATCACGG ACACGCACGT CGCGCACCAC CTCTTCTCCA 
CCATGCCGCA CTACCACGCC ATGGAGGCCA CTAAGGCGAT CCGCCCCATC 
CTCGGCGAGT ACTACCAGTT CGACCCGACG CCCGTCGCCA AGGCGACATG 
GCGCGAGGCC AAGGAGTGCA TCTACGTCGA GCCTGAGGAC AACAAGGGCG 
TCTTCTGGTA CAACAACAAG TTCTAACTGC TGCTGCTGTG AAATCAGCAT 
CACACATCCA TAGCCAAGCA GCAAACAAAT TTGAAGAAGA AGATTACAAG 
GGAAGAGAAG ATAGTGTCTT CGGAAATCGT CGTAGCAAGT ATCCATCCAT 
CCATCCAACC CATGAACAAT CGTCTATCTA TCCATGCATC TATCTATGGT 
TAGTCTCTTT AGATAGGAGA GGGCACTTGG GCACAGAGGA AGGCTATTGC 
AGTGCCATTG CTAGAGTTGC CATCAAGTGC AAAGTAGGCG GATCAGGCGT 
GTGTCATGCC TGTGGATTTG TAGTCTATGT ATGTGTCAGC TGCTGAGCTC 
CGGTGTCGCA GCCTTGGTCC CTGTCGTGTT ATTTCCATCG TTTTTTTCCC

Gene models with high sequence identity

AT3G12120.2 [+] show detail - fatty acid desaturase 2
E-value: 1e-160; Score: 563.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Fatty acid desaturase, type 1	3e-15
HMMPfam	Protein of unknown function DUF3474	3.1e-12
HMMPfam	Fatty acid desaturase, type 1	3.5e-15

See the detailed result >

AT3G12120.2

Model type: Protein coding
Short Description: fatty acid desaturase 2
Curator Summary: Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
Computational Description: fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
Link: InterPro Scan - TAIR