K18332

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18332

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18332

Determination of introduced cDNA(s)

  • J033143P19 - Rice Full-length cDNAs - Original: J033143P19

    full reading [+] show sequence

    Nucleotide sequence: J033143P19 - full reading 
    GGTGCTGTTT GCAGGCGGAC GCCGCGACGG ACGAGGTGTA CGCGCAGGTC 
    TCGTTGGTCG CCGACAACGA GGAAGTGGAG AGGCGGATGC GGGAGGGAGA 
    GGACGGCGCG GCCTGCGACG GCGAGGGCGA GGACGCCGTG AAGCGGCCGG 
    CCCGGATTCC CCACATGTTC TGCAAGACGC TCACGGCCTC CGACACCAGC 
    ACGCACGGCG GCTTCTCCGT GCCTCGCCGC GCCGCCGAGG ACTGCTTCCC 
    GCCGCTGGAC TACAGCCTGC AGAGGCCATT CCAGGAGCTC GTGGCAAAGG 
    ATCTGCACGG CACGGAATGG AGGTTCAGAC ATATCTATCG AGGCCAACCA 
    CGGAGGCACC TTTTAACCAC TGGATGGAGC GGGTTTATCA ACAAGAAGAA 
    GCTAGTATCT GGGGATGCAG TGCTATTCCT CAGAGGTGAA GATGGAGAGC 
    TTCGATTGGG AGTGCGCCGA GCTGCTCAGC TAAAAAATGC ATCTCCTTTT 
    CCTGCACTTC ATAACCAGAT CTCAAATACT AGTAGTCTTA GTGAAGTTGC 
    ACATGCTGTG GCCGTGAAAA GTATTTTTCA CATCTACTAC AATCCCAGTT 
    GTACTCACAG GTTAAGTCAG TCTGAGTTCA TTATACCATA TTGGAAGTTT 
    ATGAGAAGCT TCAGTCAACC CTTTTCTGTT GGAATGCGAT TCAAATTGAG 
    ATATGAGAGT GAGGATGCTT CTGAAAGAAG GCGTACTGGA ATAATAATTG 
    GAAGCAGAGA GGCAGATCCA ATGTGGCATG GTTCGAAGTG GAAATGTTTA 
    GTGGTGAAAT GGGATGATGA TGTGGAGTGC CGTCGGCCTA ATGGGGTATC 
    TCCTTGGGAG ATTGAGCTTA GTGGATCAGT TTCAGGATCT CATCTGTCCA 
    CTCCCCATTC AAAACGGCTG AAATCATGCT TCCCCCAAGT TAATCCAGAT 
    ATTGTGCTTC CAAATGGAAG TGTTTCTTCA GATTTTGCGG AGTCTGCCAG 
    ATTCCACAAG GTCTTGCAAG GTCAAGAATT GTTGGGTTTA AAAACTCGTG 
    ATGGTACTGT TAATACAGCT TCTCAGGCGA CTGAGGCAAG AAATTTTCAG 
    TACACTGATG AACGAAGTTG CTCTATCAAC ATGAGTAACA ATATCTTAGG 
    GGTTCCGAGA CTTGGGGTGA AAACTCCAAG TGGAAACCCT GGGTTTTCCT 
    ACCATTGCTC AGGCTTTGGG GAATCTCAAA GATTCCAAGA GGTCTTGCAA 
    GGTCAAGAAG TGTTTCGTCC TTACCGAGGA GGGACTTTGT CCGATGCCTG 
    TATAAGAGGT TCTGGCTTCC GTCAACCTGA TGGCAACCAT GCACCTGGTG 
    CAGCATTTAA ATGGCTTGCA CCACAAGGAT GTGATCATCA TGGGATAACC 
    ACATCAGTTC TGCCGCAAGC ATCCTCTCCA TCATCTGTCT TAATGTTTCC 
    ACAAACTAGT TCAAAGATGC CTGGCCTTGA ATACATATAC GGATGCCTGG 
    ATAGAAATGA GAATAGCCGA CATTTTAAGA TTGGACCTAC ACAAGATATG 
    ACAAGAACTG ATCAAACATT ACGACTCTGG CCTCATCTTA TTTCAGGCAA 
    AGTGCTAGAT GAGTGTACGA GAAATGAGAA GCTGCACTCT CCAGTCAGTG 
    GTGCTGAGCA TGAATCAAAT AACAAGTGTC TTAATACAAA TGGCTGCAAA 
    ATCTTTGGTA TATCATTGAC TGAAAAGGCC CAAGCAGGCG ATGAAGTGGA 
    TTGTGGCAAT GCCAGTTATC ATTCTCGGCT CCAGTCTTTA AAACCACAGA 
    TGCCAAAATC ATTGGGCAGC AGTTGTGCCA CGGTCCATGA ACAAAGGCCT 
    GTTGTTGGCA GGGTCGTTGA TATTTCAGCA GTGAATACGA TGATCTGATA 
    CATTTACTGT ATCCAGGGAG TATGCTTGCA GAAATAGGGA CCAACATTAG 
    TATCATACTC ACTGGTGCTG AGGACACAAC TTCCATTCTG GCTATCTCGT 
    CCCTCTACGT GCTTGTTGCC TCAACTTCAG CATTTCCACA GGCTGTGCTA 
    CCGCATCCAG GATGATGACA GGGGAAATGC AGATGATACA CGAAGGATGC 
    TTGTCAAGTT TGTTGTAATG CACTTCTAAG TTCTAAGCTT TGAACCTCTT 
    AACGTTGCTC CTATTTTAAG CTCCCGTTGT AATCGGCATT TATGTTGCCT 
    GACCTACTTG CATATTTTTC AATTTGTCCG TTAAGACAGC CCTTTTAGAG 
    GGCTTGCTTA TGTTAGCAAC CCTTAATTTC CAAATTCCAA GTATGGCGAC 
    CGTTCGAGGG GTTTGTTTGC TCCTGGGACG ACATCGGT

    Gene models with high sequence identity

    1. AT2G33860.1 [+] show detail - Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related
      E-value: 1e-112; Score: 404.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 6.8e-41
    HMMPfam Auxin response factor 8.3e-29
    HMMPfam Transcriptional factor B3 1.5e-22
    ProfileScan Transcriptional factor B3 12.901

    See the detailed result >

    AT2G33860.1

    Model type
    Protein coding
    Short Description
    Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related
    Curator Summary
    ettin (ett) mutations have pleiotropic effects on Arabidopsis flower development, causing increases in perianth organ number, decreases in stamen number and anther formation, and apical-basal patterning defects in the gynoecium. The ETTIN gene encodes a protein with homology to DNA binding proteins which bind to auxin response elements. ETT transcript is expressed throughout stage 1 floral meristems and subsequently resolves to a complex pattern within petal, stamen and carpel primordia. ETT probably functions to impart regional identity in floral meristems that affects perianth organ number spacing, stamen formation, and regional differentiation in stamens and the gynoecium. During stage 5, ETT expression appears in a ring at the top of the floral meristem before morphological appearance of the gynoecium, consistent with the proposal that ETT is involved in prepatterning apical and basal boundaries in the gynoecium primordium. It is a target of the ta-siRNA tasiR-ARF.
    Computational Description
    ETTIN (ETT); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: response to auxin stimulus, vegetative phase change, regulation of transcription, DNA-dependent, abaxial cell fate specification, auxin metabolic process; LOCATED IN: nucleus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340), Auxin response factor (InterPro:IPR010525); BEST Arabidopsis thaliana protein match is: auxin response factor 4 (TAIR:AT5G60450.1); Has 1211 Blast hits to 1200 proteins in 55 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 0; Plants - 1202; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR