K19149

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K19149

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K19149

Determination of introduced cDNA(s)

  • J013093A03 - Rice Full-length cDNAs - Original: J013093A03

    full reading [+] show sequence

    Nucleotide sequence: J013093A03 - full reading 
    GTTATTCACT CCACCCACCT CGCCGGAATG CTCGCCGTCG TCTTCTCCTC 
    CGGTCACCGC CTCCTCCCCC TCCCCGTCCT CCCCGGCACA TTCACCACCC 
    CTCCTCCTCC TCCTCCTCCT CCTCTCCTAT CCCCTCGCCG TCCTCTGCTC 
    GCCCCTCGCC GGCGCCGCTG CCTATGCGGC GGCGGCGGTG GCGGCCTCCT 
    CCTCCTTCGC GCCGTCGCCG CGAGGCGCGC GGGCATCGTG ATCGACGTCG 
    ACGAGGTGGG CGAGGTCGGC GACCGCGACC TCCCCGTGGA CGTGTCGTTC 
    ACCCGGAGGC TGCCGCCGGT GCTCACCCTC GGGGACGGCC TCGCCGCGCT 
    GCGGCGGGCC GGGGAGGAGG TGAAGGCCTG CCCGCCTGCC GCCGCCGCGA 
    GCGGCGTCAT CCGGTTCGAG GTGCTTGTTC CACCCAGTAC GAAGGCGCTC 
    AAATGGCTGT GCACACAGTT TAAGAGATCA TCATTGTTTC CTCAGTTTTA 
    TTTGTCGAGG AAGCAAACCA CTGATTCATC AATTCAGCTT GAAATCTCTG 
    GCGCTGGCTC TGCAATTTGC TTTCATGGTT CCTCTCGAGT AGATAATGGG 
    TTTGATTTGA TATCAAGATA TCTTTCGTTT AATTCACACT TGATTAGAGC 
    CTATGGATCA GTAGGTGTTA AATATGACAA GGAACTATTG TCTTTAGAGG 
    AGAGAATTGG TTCATTTTAC TTCTTTATTC CTCAGGTTGA GCTGAGTGAA 
    TTTGATGGCT ATTCCATGTT ATCGTCAACT ATTGTTTGGG ATGACTCCGT 
    GTCTCACACA TTTGAGGATT CTGTTTGTTT ATTTGAATCT TGTTTCAGTC 
    AGATATGGAG TAGCTATGAT TCTTCAGCTA CTATCTGTTA TGAAAACATG 
    GTAACAAGTT ACATTGGAGA ATCGCGTATG TCAGAGAGCA GGAATACTCA 
    GTTGGTATAC TTGGATGCTG AATTTCTCGC TGTAATAGAT GGCAAAGCTG 
    TCACGGAAAA GGTATCCTCT TAACCCTGGA GAATTGCCCA ACTTCTGATC 
    AGTCATTTGT TCGGTTTTCG CCGCAGTTCT TGTTTTGTGC AAACATGGAT 
    TTATGTTTGC AAAGCAACAA AATTGAATCT TTCATTAGGA GATGCTCTAA 
    CATTAATTTG GCATGGGCAT CCTTCATAGT TGAAGAATTT GTCAGGCTTG 
    GTTTTACGTA CTTCTGTATA GCTCCAGGGT CGAGATCATC CCCACTTGCA 
    CTTTCTGCTT CAGTCCATCC TTTGACAACC TGCATATCAT GTTATGATGA 
    GCGTTCACTT GGATTTCATG CTCTTGGCTA TGGGAGAGGT TCTCGGAAGC 
    CTGCAATAGT AATTACATCA TCAGGAACTG CTGTATCAAA TCTCCTTCCT 
    TCAGTGGTGG AGGCAAGTCA AGATTTCGTA CCACTTATTT TACTCACAGC 
    TGATCGTCCT CCTGAGCTGC AGGATGTAGG AGCCAACCAA GCCATCAATC 
    AGTTGATTCA GCAGCCTACT ATGCCATGCA AGCACCACAA GGGCCAGTGC 
    ATATAAACTG TGCTTTTAGA GAACCACTAG ACTACGGATA TCAAGATTGG 
    AGTGTTGACT GTTTGAAAGG GTTGGACAAA TGGTTTATAA ATAGAGAACC 
    ATACACTAGA TACCTAGGAA TGAAAATGGT TTCTGCATTA GGTAACTATT 
    CTTGTTCAGT AAGGGAGGTT CTGGAGATTG TAAAGAACGC AAACCAGGGG 
    CTTTTATTAG TTGGTGCTAT TCACACAGAA GATGATATCT GGGCTGTGAC 
    TTTACTAGCT AGACACCTTT CCTGGCCGAT TGCTGCTGAT GTTCTGTCTG 
    GATTGCGAAT GAGGAAAGTA CAGAAATCAA TTCCAGGACT AGATAAGAGC 
    ATTTGTTTTA TAGACCACAT AGATCAAATT CTACTGTCTG AATCTGTTAA 
    AAGCTGGAAA ACTCCAGATG TTATTGTCCA GATTGGAAGC CGGATCACTA 
    GCAAACGAGT GGGAACATAT CCTGAGTCCT GCTCCCCGTC TTCTTACATC 
    TTAATTGATG CACACCCATG CCGCCATGAT CCTTCACATG TTGTCACTCA 
    CAGAATCCAG GCTACTATAA CTGAATTTGC TGCTAGTCTG TGCCAGTGTA 
    ATTTCCAAAC AAAGACAAGC AGATGGTCAG ATATTTTAAT GGTTCTGAAT 
    TCAGCGGTTT CACAGGAGAT AATGTTTCAG GTGCATTCAG AATGTTCACT 
    TACAGAACCA TATGTTGCTC ATGTAATTGG AGAAGCACTT TATGGCGATG 
    CTACTATGTT TATTGGGAAT AGCATGGTCA TTCGAGACTT GGATATGTTT 
    GGCAAGGGCT GGATTGACCA TAGTACAAAT GCAAATAATG CTATGATGCA 
    CCATTTTCCA GGCTTTCTTG GTGCACCAGT AGCTGGGAAC AGGGGAGCAA 
    GTGGTATTGA TGGTTTGCTT AGTACATCAA TTGGATTTGC CATTGGATCA 
    AACAAGCATG TATTCTGTGT GATTGGTGAC ATATCTTTTC TTCATGATAC 
    CAATGGATTG TCACTTCTGA ACCAAAGGTT TACAGTTTGA ATCTATTCTA 
    TGCTATCCAC CACCGTTTGT TTTTCTGCGA GTCATTGGCT CATTGTCATT 
    TCCTTAATTT ATTTTTCATT GACCCAAGCA TTTGTTTTTG CATTACTACT 
    TAGTCAAAGA AATAAAAAGA ACTACAGATA TTCTCTCTGT AAAGCTATTG 
    TATAACCATC TACTTAAATG AATATTTATT TCTGATAAAG TGATAATGCA 
    TCTCACATTC CTGAAGGACG CAGAGGAAAC CCATGACAGT AATTGTCATT 
    AACAACCATG GTGGTGCAAT CTTCAGCCTT CTACCAGTTG CAAAAACAGC 
    TTCACTCCAA ATTTTGGAGA AATTTTTCTA CACCTTGCAT GACATATCGA 
    TTTCCAAACT CTGTGCTGCA CACAGGTAAA AGCGATTGTT GTATTCTGGT 
    GCTTACATAC CTCGGCCTTG CTTATAGAAA TACAATTTTA ACAATATGCC 
    TGCCTGATGC TAACGTAGCA TCGCTGTTAC TGATTCTGGG ATTTATTAGA 
    TGTTTTCTGC TGAGACTTCT GCCTGTTACA TTATTGGTTA TGGAGTCAGC 
    TGTGGATACA ACAAATAACT TTCTGGTGGC AATTTTGCAT CCCCAGCATT 
    CTCATGAGTT GTATTTCAGG ATAAAACATA TTCTAGTTCA GACAAAAGCT 
    GAACTTCATG ATGCCTTAGT GAAGTCCCAT GAGGGGCATG TTGATTGTGT 
    TGTCGAAGTG GAGAATCGCA TTGTTGATAA TGCCAACTTT CATAGGTTTT 
    ATATTCAAAC TAGTTCTTTT GATTATTTTG GTCTACATCG CGTTTATATG 
    CTCATTCTCA TTCTGCAGAA TTATAAGCAT GTTCACAGAC CATAGTGCAA 
    CCATGCATCT GGCATATCTT CTGGGAGGTC CATATTGTAA GGATGGGGTA 
    AATGGCTTTT CTGTTGGTAG AATACATGCA GCAGAATACA TGTTTTACAG 
    GATCCAACTT GCTGCACCAC GTACATCTGG GATATCAGAA AGCAGCTTCT 
    TTCATGAAGG TTTCATACTA AAGTTATGTG TGGGTGACAG CATTGTGGGA 
    TTCGGCGAGG TTGCACCAAT TGAAATTCAT GAGGAGGATC TGTTGGATGT 
    TGAAGAACAG CTTAGGTTTC TCTTTCACAG AATGAAAGAT GCTGAGCTAG 
    ATGTTGTTCC TTTGTTGAGA GGATCCTTCT CCAATTGGAT ATGGACAACC 
    CTTGGGATTC CTCCTTCTTC AGTATTCCCT AGTGTTAAGT GTGGTCTAGA 
    GATGGCTATT CTTAATTTGC TGGAGTCACA ACGAATAGAT AGATCCTATG 
    GTATTTTTAC TGGCTCCAAT GTGGTCGAAT ATAATCAGAG CAGCACTGCA 
    AACATACAGA TATGTGCACT CGTAGACTCC TGTGGCACTC CAATGGATGT 
    AACACTTGCT GTTGTCAAAC TTGTTGCTGA AGGTTTCACC ACCGTTAAAC 
    TGAAGGTTGG GCGTCGCGAA AATCCCGCTG AAGATGCAGC TGTTATTCAA 
    AAAGTAAGGG AAATTGTAGG ATACAAGATC AATATCCGTG CTGACGCAAA 
    TAGGAAATGG ACATATGAAC AGGCAATTGA TTTTGGATCC AGGGTAAAAG 
    GCTTATGTTT GCAATACATC GAGTTCTTCC TTGAGAGTCT TCATTTGTTT 
    TAACCACAGG AACCAGTGGA CTCTGTAAAT GACATCATCA AGTTCTGTGA 
    AAATAGTGGC TTGCCTGTTG CACTAGACGA GACTATCGAC AACCTTACAG 
    GGGATGTAAT CCCTAAGCTA CATCAATTTT CACATCCAGG AATAGTTGCT 
    CTTGTTATAA AACCCAGTGT TGTTGGTGGC TTTGAGACTG CAGCTTATAT 
    AGCAAAATGG GCTCACATGC ATGATAAGAT GGCTGTCATA GTAGCACCTA 
    TGAGAGTTCT GTAGGTTTGG CAACCTATAT ACAGTTTGCA CATTATGTTG 
    ACAGACAAAA TGACATAACA TCCAGAATAA AGAACAGAGG TTCTTGTGGA 
    AATGTGGCAC ATGGACTTGG AACATACCAA TGGTTAAGAG AAGATGTTTC 
    AGATCAAAAG TTAAAAATCC ATGCGCCACC ACTTGGTGAT GGAATCAGAG 
    CTTCGGC

    Gene models with high sequence identity

    1. AT1G68890.1 [+] show detail - magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyru...
      E-value: 1e-155; Score: 205.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 4.5e-24
    Gene3D no description 2.6e-13
    HMMPanther Mandelate racemase/muconate lactonizing enzyme 1.5e-08
    HMMPanther MENAQUINONE BIOSYNTHESIS PROTEIN 5.1e-48
    HMMPanther THIAMINE PYROPHOSPHATE ENZYMES 5.1e-48
    HMMPanther MENAQUINONE BIOSYNTHESIS PROTEIN 2.4e-05
    HMMPanther THIAMINE PYROPHOSPHATE ENZYMES 2.4e-05
    superfamily DHS-like NAD/FAD-binding domain 5.6e-13
    superfamily Thiamin diphosphate-binding fold (THDP-bindin 1.1e-07
    superfamily Thiamin diphosphate-binding fold (THDP-bindin 1.1e-20
    superfamily Enolase C-terminal domain-like 1.7e-21
    superfamily Enolase N-terminal domain-like 2.1e-08
    superfamily Enolase C-terminal domain-like 3.8e-10
    HMMPfam Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain 4.1e-21
    HMMPfam Mandelate racemase/muconate lactonizing enzyme, C-terminal 1.9e-14

    See the detailed result >

    AT1G68890.1

    Model type
    Protein coding
    Short Description
    magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases
    Curator Summary
    Homologous to the four eubacterial men genes involved in menanoquinone biosynthesis. Studies of mutants defective in this gene demonstrated its involvement in phylloquinone biosynthesis in Arabidopsis.
    Computational Description
    magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases; FUNCTIONS IN: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity, hydro-lyase activity, magnesium ion binding, thiamin pyrophosphate binding, catalytic activity; INVOLVED IN: phylloquinone biosynthetic process, photosystem I stabilization; LOCATED IN: chloroplast; EXPRESSED IN: shoot, leaf whorl; CONTAINS InterPro DOMAIN/s: Menaquinone biosynthesis protein MenD (InterPro:IPR004433), Alpha/beta hydrolase fold-1 (InterPro:IPR000073), Mandelate racemase/muconate lactonizing enzyme, C-terminal (InterPro:IPR013342), Thiamine pyrophosphate enzyme, C-terminal TPP-binding (InterPro:IPR011766), Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain (InterPro:IPR012001), O-succinylbenzoic acid (OSB) synthetase, gamma proteobacteria/archaea (InterPro:IPR010196), Mandelate racemase/muconate lactonizing enzyme, conserved site (InterPro:IPR018110); BEST Arabidopsis thaliana protein match is: isochorismate synthase 2 (TAIR:AT1G18870.2); Has 22452 Blast hits to 22188 proteins in 2448 species: Archae - 403; Bacteria - 16894; Metazoa - 237; Fungi - 200; Plants - 484; Viruses - 7; Other Eukaryotes - 4227 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR