K21735

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K21735

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21735

Determination of introduced cDNA(s)

  • J013134I09 - Rice Full-length cDNAs - Original: J013134I09

    full reading [+] show sequence

    Nucleotide sequence: J013134I09 - full reading 
    GACAGCCATC TCCTCTCTAC TGTCAATCTC AAGTGCGTGC CCGGTATCAG 
    CTCGACCGGT CTCGCACCTG CAACCAAACC ACACACAGCT AGCTATCATG 
    GCCTCTAAGC CAGGGCCTCT CACTCAGTGG CCGTGGCATA ACCTTGGAAA 
    CTACAAGTAC GCGCTGGTGG CGCCGTCGGC GGCGTACAGC ACGTACAGGT 
    TCGTGACGGC GAGCAGCGCG GCGGAGCGAG ACCTCCTCAA CTTCATGGTG 
    TTCCCCATGC TGCTCCTCCG TCTGCTCTAC GGCCAGCTCT GGATCACCGT 
    CTCCCGCCAC CAGACTGCCC GGAGCAAGCA CAAGATCGTC AACAAGAGCC 
    TCGACTTCGA ACAAATCGAC AGGGAAAGAA ACTGGGATGA CCAGATAATC 
    CTGACGGCGC TGGTGTTCTA CTTGGTGAGC GCGACGATGC CGCAGGCGCA 
    GGTGGCACCG TGGTGGAGCA CAAAGGGGAT GGTGGTGACG GCGGTGCTGC 
    ACGCGGGGCC AGTGGAGTTC CTCTACTACT GGCTGCACAG GGCGCTGCAC 
    CACCACTGGC TGTACGCGCG GTACCACTCG CACCACCACG CCTCCATCGT 
    CACTGAGCCC ATCACCTCCG TCATCCACCC CTTCGCCGAG GAGGTCGTCT 
    ACTTCGTCCT CCTCGCCATC CCCATCCTCA GCACCGTCGC CACCGGCACC 
    GTCTCTGTCG TCACGGCCAA CGGCTACCTC GTCTACATCG ACTTCATGAA 
    CTACCTCGGC CACTGCAACT TCGAGCTCGT CCCCAAGTGC CTCTTCCACG 
    TCTTCCCCCC TCTCAAATAT CTCCTCTACA CCCCATCGTT TCATTCGTTG 
    CACCACACGC AGTTCAGGAC GAACTACTCG CTGTTCATGC CGGTGTACGA 
    CTACATCTAC GGGACGACGG ACAAGTCAAG CGACGAGCTG TACGAGAGGA 
    CGCTGCAGGG GAGGGACGAG GCGGCGTGGC GGCCGGACGT GGTGCACCTC 
    ACGCACCTCA CCACGCCGGA GTCCGTCTTC CACAACCGCC TCGGCTTCGC 
    CGCCGTCGCG TCCAACCCGC TTGGCGCCGC AGCCTCCGGC CACCTCCTCC 
    GTGCCGCCTC CGCCGTCGCC TCCCCTCTCC TCTCCCTCTT CGCCTCCACC 
    TTCAGGTCGG AGGCCAACCG CCTCGACAAG CTCAACATCG AGACCTGGGT 
    CATCCCAAGG TTCACCTCCC ACTATACGTC GAAGAGTGAT GGGTACAAGG 
    TCAGCCGTTT GATAGAGAAA GCGGTGTCAG ACGCAGAAGC AAGTGGCGCG 
    AGAGTCCTCA CGCTCGGCCT CCTCAACCAG GGGTACGATC TGAACAGGAA 
    CGGGGAGTTA TATGTCGTCA GGAAGCCAAG CCTGAAGACG AAGATCGTCG 
    ATGGAACAAG CCTCGCTGTT GCTGCCGTTC TCAACATGAT CCCTCAGGGC 
    ACCAAAGATG TGCTCCTCCT GGGAAACGCG AACAAGATCT CTCTCGTTCT 
    TACCCTGTCC CTCTGCAAGC GGGAGATCCA GGTGAGGATG GTAAATAAGG 
    AGCTGTATGA ATGCCTGAAG CAGCAGCTGC AGCCTGAAAT GCAGGAGCAT 
    CTGGTTTTGT CATGCAGTTA CAGCAGCAAG GTGTGGCTCG TGGGCGACGG 
    GGTGACGGAT GAGGAGCAGA TGAAAGCCCA GAAAGGATCC CATTTCGTGC 
    CGTACTCGCA GTTCCCTCCC AACAAGGCCC GCAACGACTG CGTCTACCAC 
    TGCACCCCGG CTCTACTCGT CCCTGAATCC TTCGAAAACC TTCACGTTTG 
    CGAGAATTGG TTGCCGAGGA GGGTGATGAG CGCGTGGCGG GCGGCGGGGA 
    TCGTTCACGC GCTGGAGAAG TGGGACGGGC ACGAGTGCGG CGGCAGGGTC 
    ACCGGCGTGC AGAAGGCGTG GAGCGCGGCG CTGGCACGGG GATTCCGGCC 
    ATACGACGAC CACCACCACC CCGGCATCAC CCATGATGGC CGTGGCGGGC 
    TGTGACCGGC CGGTGGCCGG ATCGAAGCAA CCTGGCCTGA TTGTGTGATC 
    TTGTGTGTGC TTCTTCGCTC GGTTGATATA TAGGAGTATC TCCGTGTGTG 
    TTATGATGGG GCCAGATTAA TATCCAGCTA TAAAATCCCA AGCTGTATTG 
    TTAATTCTCT GCCGGTGTCA TAATCTATTG TTTGAGCATA ACTTAAATTT 
    GAGCATGATT AATTTGGAG

    Gene models with high sequence identity

    1. AT1G02205.2 [+] show detail - Fatty acid hydroxylase superfamily
      E-value: 0; Score: 701.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Uncharacterised protein, Wax2 C-terminal 4.4e-74
    HMMPfam Fatty acid hydroxylase 1.3e-13
    HMMPanther STEROL DESATURASE 2.3e-149
    HMMPanther STEROL DESATURASE 2.3e-149

    See the detailed result >

    AT1G02205.2

    Model type
    Protein coding
    Short Description
    Fatty acid hydroxylase superfamily
    Curator Summary
    Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
    Computational Description
    ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR