K21840

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K21840

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21840

Determination of introduced cDNA(s)

  • J023060M02 - Rice Full-length cDNAs - Original: J023060M02

    full reading [+] show sequence

    Nucleotide sequence: J023060M02 - full reading 
    TTGATGAACG TGTTGACTGA TTGCTTCGTG TAATTGCGGT CAGCAGCAGT 
    ACGTATGGGT ACCAGCAGCC GGCCGACGAC GGTGAAGGAG GGGAAGAAAC 
    TAGAGGCGCC CCGCCGTGCC GGCAGCCATG CAGCCGTGAA GCGCTCTCCG 
    GTGGACAAGC CGCCGTTCAC GCTGGGCGAC ATCAGAAAGG CCATCCCACC 
    GCACTGCTTC CACCGCTCCG TGATCAAGTC ATTCTCCTAC CTGCTCCACG 
    ACCTTGCCAT TGCCGCGGGC CTCCTCTACT TTGCTCTGGT CGTCATCCCT 
    GCCCTCCCAG GCGTCCTCCG CCTCGTCGCC TGGCCGTTCT ACTGGGCCGC 
    GCAGGGGTGC TTCCTGTTCG GGGTGTGGAT CATCGCGCAC GAGTGCGGGC 
    ACCACGCGTT CTCGGGCCAC GCACTCCTCG ACGACACCCT CGGCCTGGTC 
    CTGCACTCAT GGCTCCTAGC GCCATACTTC TCGTGGAAAT ACACCCACCA 
    ACGGCACCAC TCCAACACCA GCTCACAGGA GCGCGACGAG GTGTTCGTCC 
    CCAGGTTCAA GTCCGACCTG CCGTGGTACT CCCCATACGT GTACAAGTAC 
    AACAACCCCG TCGCTCGGCT GCTGCTCCTC GTCGTGCAGC TCACCGTCGG 
    GTGGCCGATG TATTTGGTGT TCAACACCTG GGGTCGCCAG TACCCAAGGT 
    TCGCCAGCCA CTTCGATCCC TCTGGGCCCA TCTACAAGGG GCGGGAGCGC 
    GTCTTCATCG CCATCTCGGA CATCGGCATG CTGGCCGTGT CGCTCGCGCT 
    GTACAGGCTT GCGGAGGGTT ACGGGTTTTG GTGGGTGGTG CGCGTCTACG 
    GCGTGCCGCT GCTTGTCGTC AACGCGTGGC TTGTGGTCGT CACGTACCTG 
    CATCACACTC ACCGGGCGAT CCCACACTAC GACTCCAGCG AGTGGGACTG 
    GTTGCGCGGG GCGCTCGCCA CCGTGGACCG CGACTACAGC TTCCTTAACC 
    GAGTGTTTCA CAACATCACG GACACACACG TCGTGCACCA CCTGTTCCCT 
    ACCATCCCGC ACTACCACGC TGTGGAGGCG ACCAAGGCGA TCCGCCCTAT 
    CCTCGGCGAG TACTACCAGT TCGATCCCAC ACCCATCGTC AAGGCGATAT 
    GGCGCGAGGC TAAGGAGTGC ATCTACATCC AGTCCGAGGA CCACAAGGGC 
    GTCTTCTGGT ACAGCAACAA GTTCTAGTAC CTGTAGCAGC GGCAAGCGGG 
    CGTATAGGAA GGGCTGACAG AATTAGCCAT GTCGGTCTTG CCTCATCGCT 
    TCATCTAAAC CCATAATCTA CTTGAAGTCT TGAATTAATT GTAAACTCAA 
    ATGAAATTCC C

    Gene models with high sequence identity

    1. AT3G12120.2 [+] show detail - fatty acid desaturase 2
      E-value: 8e-136; Score: 480.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Fatty acid desaturase, type 1 1.2e-29
    HMMPfam Protein of unknown function DUF3474 1.5e-10

    See the detailed result >

    AT3G12120.2

    Model type
    Protein coding
    Short Description
    fatty acid desaturase 2
    Curator Summary
    Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
    Computational Description
    fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR