K23025

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K23025

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K23025

Determination of introduced cDNA(s)

  • J023108I12 - Rice Full-length cDNAs - Original: J023108I12

    full reading [+] show sequence

    Nucleotide sequence: J023108I12 - full reading
    GACCCGCTCT CTCCTCTCAT CTCCTCCTCC TGTGTTCGTG TTCTTCTTCT 
    TCTTGTAGCA GCGCTTAACC AACCCATCAT TAGCGACCAA ACCTCTCTCC
    CTCTCCCCCT CTGACTAATC AAAATTAAAC AATTTCGATC TCCACCTTCC
    TCTTCTTGAT TCCAGTTCCA CCAGAGAGAG ACAGGGTCTT CTCTAATCCA
    TGTGGTGCTT GTTGGTCTCG CTTGCTTCGT CTTCGTCTTC GTCTTCCTCT
    TCTCGCACCT AGTCCCGCCT CGTCGCCTCC TCCGGAGACC GGAGCCGGAC
    GGGACGGTTG CCGGCGAGCG GCGGTCGCTT TCTTCTCTCG GCCCTACTCA
    CGTTATTTTC AGTGACTCTT CTTCCAAACC AAGTGGTAAA GCCAGGATTC
    TACCATTACA ATGACGATCT GCAGCTGTGA GGAGACGATC AATGAGTTTG
    AGATGTTAAC GCGTGATGCT GCGCGTGTGC AGAAGGATAC ACTGAAAAAG
    ATCCTTGAGA TAAATGCTAG TGCTGAGTAC CTGCAGAACT TTGGGCTTGG
    GGGGAGGACT GATGCCGAGA GCTACAAATC CTGCATCCCA TTGTGTGTGC
    ACAATGATAT TGAGCCCTAT ATCCAGCGGA TTGTTGATGG CGATACCTCA
    CCAGTGGTCA CTGGAGAACC CATCACCAAC CTCTCTCTCA GTTCCGGTAC
    AACACACGGA AAGCCCAAGT TCATTCCATT CAATGATGAA TTACTTGAGA
    CCACACTTCA AATATACCGT ACATCTTACG CGTTTAGGAA CCGTGAATAC
    CCTATTGGCC AAGGAAAAGC ACTGCAGTTT GTTTATGGTA GCAAGCAAGT
    CATAACGAAA GGTGGCATTC TTGCAACAAC TGCGACAACA AACTTGTATA
    GGAGACAGCG CTATAAGGAA GGCATGAAGG ATATCCAGTC GCAGTGTTGC
    AGTCCTGATG AAGTTATCTT TGGCCCTGAT TTCCACCAAT CCTTGTACTG
    TCACTTGCTG TGTGGGTTAA TATACTCAGA GGAGGTCCAT TCAGTGTTCT
    CAACATTTGC TCACAGCCTA GTCCATGCAT TCCAAACATT CGAGGAGGTT
    TGGGAAGATC TATGCACTGA TATAAGAGAC GGTGTTCTCT CAAAGAAAGT
    TACAGCACCA TCAATTCGTG AGGCTGTTTC AAAAATCCTG AAGCCCAATC
    CTGAACTTGC TGACTCAATA TACAAGAAGT GTATTGGTTT GAGCAATTGG
    TATGGAGTGA TCCCAGCACT GTGGCCAAAT GCAAAGTATG TGTATGGTAT
    CATGACAGGA TCCATGGAGC CATATCTGAA AAAATTAAGG CATTACGCAG
    GGAACTTGCC ACTTATAAGT GCTGACTATG GCGCATCGGA AGGATGGGTT
    GGCTCTAACA TAGACCCTAC TGTGCCACCA GAGCAGGTGA CATATGCTGT
    TCTCCCACAA GTTGGTTATT TTGAGTTCAT TCCTTTGGAG AAACCAATAG
    GGGAGGAGAC AGAAAACTCC GCCTCCATTC ATTACATAGA ATCAGATCCA
    GTTGGCTTGA CTGAAGTTGA GGTCGGCAAA ATCTATGAAG TTGTAATAAC
    TAACTTTGCA GGTCTATATC GGTACAGGCT AGGAGATGTC GTGAAGATAG
    CACGCTTCCA CAACTCCACA CCTGAGCTCC AATTCATCTG TCGCAGAAGC
    CTAGTGCTGA GCATCAACAT CGACAAGAAC ACCGAGAAAG ACCTCCAGTT
    GGCTGTTGAG GAGGCATCAA AGTTCCTGGA AGGAGAGAAG CTCGAAGTCA
    TGGATTTCAC GAGCTTCGTG GAACGATCAA GCGATCCAGG GCGCTACGTG
    ATCTTCTGGG AGCTGAGTGG CGATGCGAGT GATGAGGTCT TGAGCAGCTG
    CGCAAATGCC TTGGATCTAG CCTTCATTGA CGCAGGCTAC ACGGGCTCGA
    GGAAGATCAA GACGATCGGC CCCCTTGAGC TCCGGATCCT CAGAAAGGGA
    ACCTTCAAGG AGATCCTAGA TCATTTCCTA AGCCTTGGTG GTGCGGTGAG
    CCAGTTCAAG ACACCAAGGT TTGTGAACCC ATCAAACAGC AAGGTGTTGC
    AGATACTGAG TAGAAATGTC ACCCAAAGTT ATTTCAGTAC TGCCTATGGA
    TTTTGATACA AAGAAACATA TTAGATGCTA GCAGCTGCAT GTAATTACCT
    TTTTACCTCT TGTTTTTCAG CTTTACTGTA GTCTCCACCG GAAGAAAACG
    TTTGGTTTAG TCTCTTGAAT AATCTATTTT CTTATCCTGT TTGTGACTAT
    CTAGAATCTA TGTAAATGGC CCAGCTCTAG TGAAAGTTAT GGTGATGTTA
    TCAGGAAAGA AAATGTGGTG TTTCCCGTAA CAAGTGCTGC AAAGTATTAT
    TCAGACTCCC

    Gene models with high sequence identity

    1. AT2G46370.4 [+] show detail - Auxin-responsive GH3 family protein
      E-value: 0; Score: 743.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam GH3 auxin-responsive promoter 5.7e-179
    Coil coiled-coil 0

    See the detailed result >

    AT2G46370.4

    Model type
    Protein coding
    Short Description
    Auxin-responsive GH3 family protein
    Curator Summary
    Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.
    Computational Description
    JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1).
    Link
    InterPro Scan - TAIR