K24232

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K24232

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K24232

Determination of introduced cDNA(s)

  • J023010O04 - Rice Full-length cDNAs - Original: J023010O04

    full reading [+] show sequence

    Nucleotide sequence: J023010O04 - full reading 
    GATCCTCCTC TCCTCTTCTC GCTCTCACTT CTTGTGCCCA AGTGTGAGAG 
    CAATGGGAGT TCTTGGTAGT AGGGTCGCTT GGGCATGGCT GGTCCAGCTG 
    CTGCTGCTCC AGCAGCTCGC CGGAGCGTCG CACGTCGTCT ACGACGACCT 
    CGAGCTGCAG GCGGCTGCTA CCACAGCGGA CGGCGTGCCG CCGTCCATCG 
    TCGACTCTGA GCTCCGGACT GGGTATCACT TCCAGCCACC CAAGAACTGG 
    ATCAATGATC CGAACGCGCC GATGTACTAC AAGGGGTGGT ACCATCTGTT 
    CTACCAGTAC AACCCCAAGG GCGCCGTGTG GGGGAACATC GTGTGGGCGC 
    ACTCAGTGTC ACGTGACCTC ATCAACTGGG TGGCGCTCAA GCCGGCCATC 
    GAGCCCAGCA TCAGGGCCGA CAAGTACGGC TGCTGGTCGG GGTCGGCGAC 
    GATGATGGCC GACGGGACGC CGGTGATCAT GTACACCGGC GTCAACCGCC 
    CCGACGTCAA CTACCAGGTG CAGAACGTGG CGCTGCCGAG GAACGGGTCG 
    GACCCGCTGC TGCGCGAGTG GGTGAAGCCC GGCCACAACC CGGTGATCGT 
    GCCCGAGGGC GGCATCAACG CGACGCAGTT CCGCGACCCG ACCACCGCGT 
    GGCGCGGGGC CGACGGCCAC TGGCGGCTGC TCGTCGGCAG CCTCGCGGGG 
    CAGTCCCGCG GCGTGGCGTA CGTGTACCGG AGCAGGGACT TCCGGCGGTG 
    GACGCGCGCG GCGCAGCCGC TGCACTCGGC GCCCACGGGG ATGTGGGAGT 
    GCCCGGACTT CTACCCGGTC ACCGCGGACG GCCGCCGCGA GGGCGTCGAC 
    ACCTCGTCCG CCGTCGTCGA CGCCGCCGCC TCGGCGCGCG TCAAGTACGT 
    GCTCAAGAAC AGCCTCGACC TGCGCCGGTA CGACTACTAC ACCGTCGGAA 
    CGTACGACCG GAAGGCCGAG CGGTACGTGC CGGACGACCC CGCCGGCGAC 
    GAGCACCACA TCCGCTACGA CTACGGCAAC TTCTACGCCT CCAAGACGTT 
    CTACGACCCG GCGAAGCGCC GCCGCATCCT CTGGGGATGG GCCAACGAGT 
    CCGACACCGC CGCCGACGAC GTGGCCAAGG GCTGGGCCGG AATCCAGGCG 
    ATTCCGAGGA AAGTGTGGCT GGACCCAAGT GGGAAGCAAC TGTTGCAGTG 
    GCCAATCGAG GAGGTCGAGA GGCTGAGAGG GAAGTGGCCG GTCATTCTCA 
    AGGACAGGGT GGTCAAGCCA GGGGAACACG TCGAGGTGAC CGGGCTACAA 
    ACTGCACAGG CTGACGTGGA GGTGAGCTTC GAGGTGGGGA GCCTGGAGGC 
    GGCGGAGCGG CTGGACCCGG CGATGGCGTA CGACGCGCAG CGGCTGTGCA 
    GCGCGCGGGG CGCCGACGCG AGGGGCGGCG TGGGGCCGTT CGGCCTGTGG 
    GTGCTCGCGT CCGCGGGGCT GGAGGAGAAG ACCGCCGTGT TCTTCAGGGT 
    GTTCAGGCCG GCGGCGCGCG GCGGCGGCGC CGGCAAGCCC GTCGTGCTCA 
    TGTGCACCGA CCCCACCAAG TCATCGCGCA ACCCGAACAT GTACCAGCCG 
    ACGTTTGCAG GGTTCGTTGA CACGGACATC ACCAACGGGA AGATATCTCT 
    GAGGAGCCTG ATCGACAGGT CGGTTGTTGA GAGCTTCGGG GCTGGAGGAA 
    AGGCGTGCAT CCTGTCGAGG GTGTACCCGT CGCTGGCCAT CGGCAAGAAC 
    GCGCGCCTTT ACGTTTTCAA TAACGGGAAG GCGGAGATCA AGGTGTCGCA 
    GCTCACCGCG TGGGAGATGA AGAAGCCGGT CATGATGAAT GGAGCCTAAA 
    CAATATTTGA AATTGAGAGA GATAGATGCA ATGCATGATG AGAACTACCT 
    TCAGTAGCTA GCTAGATTTT TGAGTTTCAG CGGAAAAGAA AAAACTGATT 
    GCCCTTAATT ATGTGCTAAA TCATGCCCCC TTGTGTAAAA TGGTTTCAGT 
    CACACGTGTC GTATGCATAT GTACCAAGGG GACAGTTTGA ATGCATGGAT 
    GCCATTAGGA CTAATAAAGA TTGATGCTAG TATAACCCT

    Gene models with high sequence identity

    1. AT2G36190.1 [+] show detail - cell wall invertase 4
      E-value: 7e-172; Score: 601.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Arabinanase/levansucrase/invertase 5.2e-99
    superfamily Concanavalin A-like lectin/glucanase 6.4e-08
    PatternScan Glycoside hydrolase, family 32, active site 0
    HMMPfam Glycosyl hydrolases family 32, N-terminal 3.4e-113
    HMMPfam Glycosyl hydrolase family 32, C-terminal 6e-19
    HMMSmart Glycoside hydrolase, family 32 7.6e-235

    See the detailed result >

    AT2G36190.1

    Model type
    Protein coding
    Short Description
    cell wall invertase 4
    Curator Summary
    cwINV4 appears to function as a cell wall-localized invertase (that can catalyze the hydrolysis of sucrose into fructose and glucose) based on the phenotype of cwinv4 mutants. cwINV4 transcripts are expressed at high levels in lateral and median nectaries and this enzyme plays an important role in nectar production.
    Computational Description
    cell wall invertase 4 (cwINV4); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 32 (InterPro:IPR001362), Glycoside hydrolase, family 32, active site (InterPro:IPR018053), Glycosyl hydrolases family 32, N-terminal (InterPro:IPR013148), Glycosyl hydrolase family 32, C-terminal (InterPro:IPR013189), Concanavalin A-like lectin/glucanase (InterPro:IPR008985); BEST Arabidopsis thaliana protein match is: cell wall invertase 2 (TAIR:AT3G52600.1); Has 4207 Blast hits to 4168 proteins in 1243 species: Archae - 18; Bacteria - 2607; Metazoa - 78; Fungi - 284; Plants - 1032; Viruses - 0; Other Eukaryotes - 188 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR