K24547

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased width (PATO:0000599) (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K24547

Determination of introduced cDNA(s)

J013091G23 - Rice Full-length cDNAs - Original: J013091G23

full reading [+] show sequence

GGAGATATAT TTTCCCCTCG GCGGCGACTC CTCCTCCACC TCGTGAGCTC 
GCCTTCGGCC GCCGCCTCTG CGCCTCGCAG ATCCCCACCC GCGACCGAGC 
GAGCTGGTGC CGCATCGGTA TCGCATCCCG TTCATCTTCT GGCTGGACGA 
ATTCAATTCA CGGACGGTGG TGGTGGTGGT GTTGACTAGG GATTTATTTT 
TTTACCATTG GCGGCTCGGC TGCATCCCCA AGTGAAGAAG AGAATTCGTA 
CATGATTCCG CTAAACAGGA GGGCGAGTCA GACTAGGGGT GGGATGGAGT 
ACTTCGATGC TAGGCGGAAG CCTCATAATG TTGGGAAAGT TATTGCAGCT 
CTGGTCCTCA CAACACTATG TATATTTATT TTGAAGCAAT CTCCTGGTTT 
TGGTGGCAGT AGTGTGTTTT CTCGCCATGA ACCTGGTGTT ACTCATGTCT 
TAGTAACAGG AGGAGCTGGC TATATTGGGT CACATGCTTC ATTACGCCTG 
TTAAAGGACA ATTATCGAGT TACCATTGTG GACAATCTTT CTAGAGGAAA 
CATGGGAGCA GTAAAGGTTC TCCAAGAGTT GTTCCCCCAG CCAGGAAGAC 
TTCAGTTTAT CTATGCTGAT CTTGGAGATC AAAAAACTGT CAACAAGATC 
TTTGCCGAAA ATGCGTTTGA TGCTGTTATG CACTTTGCTG CTGTTGCCTA 
TGTGGGTGAG AGTACATTGG AACCCCTTAG GTACTATCAC AATATTACAT 
CGAACACCTT ATTGATTTTG GAGGCTATGG CGTCTCATGG AGTCAAGACC 
CTTATTTACT CTAGTACATG TGCCACCTAT GGAGAACCAG AGAAGATGCC 
TATTGTAGAA ACCACACGTC AGTTACCAAT TAATCCCTAT GGAAAAGCTA 
AGAAAATGGC GGAGGACATC ATACTAGATT TCACGAAGGG GAGAAAGGAC 
ATGGCTGTGA TGATTTTAAG ATATTTCAAT GTTATTGGAT CAGACCCAGA 
GGGAAGACTA GGTGAAGCTC CTAGACCTGA ACTACGAGAG CATGGAAGGA 
TATCCGGGGC ATGCTTTGAT GCTGCGTTAG GAATCATTCC AGGGCTGAAG 
GTTAAAGGAA CAGATTATCC TACAACTGAT GGAACCTGCA TAAGAGACTA 
CATTGATGTC ACAGATCTTG TTGATGCTCA TGTGAAAGCA CTCAACAAGG 
CAGAGCCAAG TAAAGTTGGC ATTTACAATG TTGGCACTGG AAGAGGTCGT 
TCAGTCAAGG AGTTTGTGGA TGCCTGCAAA AAGGCAACTG GAGTCAACAT 
TAAAATTGAG TACCTCAGCA GGAGGCCAGG GGACTATGCT GAAGTATACG 
GTGACCCAAC AAAGATCAAC ACGGAGCTCA ACTGGACTGC TCAATATACC 
GACCTCAAGG AGAGCCTGTC AGTCGCTTGG AGATGGCAAA AGTCACATCC 
ACGCGGATAT GGGTCAAACT AAATATAGCT TGCTGATCAA TAATTCTGCT 
GAATCGTCGA TATATTGATT TTTTTAGTTA GTGGTATAGG ATCTTGGTGC 
GATACCGCTT CCACAGTCAC TCATTTGTTA TTTTTTTCTC TTTTTATTTT 
CAAAGGTTTG TTGTTATTTT CTAGCAAAAC CATAAGCAGT GATCTTGATA 
TGCTGGAAAT CCTGGAAGCC TTCCTGTAAG GGCAAATTTC ATTGTTTTTT 
GACACAAATT GCTGATAAAA AGAAGTGTAC ATTGTCTCTT ACC

Gene models with high sequence identity

AT1G30620.2 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
E-value: 0; Score: 647.00

InterPro Scan Digest

Program	Description	E-value
HMMTigr	UDP-glucose 4-epimerase	2.6e-142
HMMPfam	NAD-dependent epimerase/dehydratase	2.3e-48
superfamily	NAD(P)-binding domain	3.8e-89
HMMPanther	UDP-glucose 4-epimerase	2.8e-149
HMMPanther	NAD DEPENDENT EPIMERASE/DEHYDRATASE	2.8e-149
Gene3D	NAD(P)-binding domain	3.3e-56

See the detailed result >

AT1G30620.2

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Curator Summary: encodes a type-II membrane protein that catalyzes 4-epimerization of UDP-D-Xylose to UDP-L-Arabinose in vitro, the nucleotide sugar used by glycosyltransferases in the arabinosylation of cell wall polysaccharides and wall-resident proteoglycans.
Computational Description: MURUS 4 (MUR4); FUNCTIONS IN: UDP-arabinose 4-epimerase activity, catalytic activity; INVOLVED IN: plant-type cell wall biogenesis, arabinose biosynthetic process, nucleotide-sugar metabolic process; LOCATED IN: Golgi apparatus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040), UDP-glucose 4-epimerase (InterPro:IPR005886); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G20460.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR