K35823

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35823

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35823

Determination of introduced cDNA(s)

  • J013020G01 - Rice Full-length cDNAs - Original: J013020G01

    full reading [+] show sequence

    Nucleotide sequence: J013020G01 - full reading 
    GAGGCTCCAG TAGCTAGCCA GTCCAGTCCA AGAGATCGAT CGAGAAACAG 
    AGGATCGGAG AGGAGGAAGA AGGAGAATCC AAGTACTGGG AGGCAATTAA 
    TTTGAGTTGA GATCGACTTG AGAGCTAGCA GAGGCTCATC TCATCTAGGC 
    CAGCAGAGGC TCTTCGCTCG CTTATTTCAT CCATGGCTTC CAAAGGCACA 
    GAAGGAGGCC ATGGTGGCGT GGAGAGAAAG GAGCAGCAGC AGCAGAGGGG 
    TTACCAGCTG CCCCGTGACT CCCGGGGATC ACTCGAGGTG TTCAACCCCT 
    CATCCGCTTC CTCCTTCCGC ACTGCTGCTG CCGCCCCCAA GTCCGCCTCC 
    CCCTTCCTTG CTATTCCCGA CAGGGAAGAG GATAATGTGG TGGCACAGCA 
    GCGGGCAGCA GAGTGGGGCC TCGTCCTCCA GACCGACCAC CACACAGGCC 
    TCCCCCAGGG CGTCTCCGCA CGACCCTCCT CTGGCTCTGC ACGCACCAGC 
    TCTGAGGACA ACCCACAGCA ACAGCAGAGC GCCGCCGCCA TCCCACGGGT 
    GTCGGAGGAG CTACGGGCGG CACTATCAGT GTTCCAACAG ACGTTTGTGG 
    TGTCTGATGC CACCCACCCC AACCACCCCA TCATGTACGC CAGCGCTGGC 
    TTCTTCAACA TGACCGGCTA TACCTCCAAG GAGGTTGTCG GCAGGAACTG 
    CCGCTTCCTT CAAGGCTCTG GCACCGACCC CCATGAGATT GACAAGATCA 
    GGCAATCCCT CGCAAATGGC TCCAACTATT GTGGCCGCAT CCTCAACTAC 
    AAGAAGGACG GCACGCCATT CTGGAACCTC TTAACCATTG CCCCTATCAA 
    GGATGAGGAC GGCAGGCTCC TCAAGTTCAT CGGGATGCAA GTGGAAGTTA 
    GTAAATACAC TGAAGGGAAA AAGGATACTG TTGTTCGTCC AAATGGACTT 
    TCAGAATCAC TCATCAAATA CGATGCTCGA CAGAAGGATC ACGCCCGTAG 
    CTCAGTGTCT GAGCTCTTGT TGGCTCTCAA AAATCCACGC TCATTGTCAG 
    AATCAAGCAA TAACACCTTA AAAAGAAAAT CACAAGAATC CCTAAGCATG 
    TCAATGAGCG AAGTTCCTAG TAAGAGAAGC TCTGAAAGTG GATCTCGCCG 
    AAATTCTCGT AGCGGAACAA GGAGCTCGCT TCAGAAGATC AATGAAGTAC 
    CTGATCAAGT GAATAGAACT AGAAAATCTG GTCTGCGTGC ATTTATGGGT 
    TTTCTTGGTA TGGGCCATGG AAGTGTAGAG AAGAACATGC TGAAACCTAG 
    AGATGAAGAT CCACTGATCG ATAGTGATGA TGAAAGACCT GAGAGCTTTG 
    AAGATGAGTT TAGGAGGAAA GAAATGAGGA GGGGTATAGA CTTGGCTACT 
    ACACTTGAAC GTATTGAGAA GAATTTTGTC ATCACAGATC CAAGGTTGCC 
    TGATAATCCC ATTATATTTG CATCCGATAG CTTTCTGCAA TTGACAGAAT 
    ACAACCGTGA AGAAATATTA GGGAGAAACT GCAGGTTTCT TCAAGGCCCT 
    GAAACTGATC GTGCAACAGT TAGGAAGATA AGAGATGCCA TAGATAACCA 
    GGCAGAGGTC ACAGTTCAGC TCATAAATTA TACAAAAAGT GGTAAAAAGT 
    TTTGGAACCT CTTTCACTTG CAACCAATGC GTGATCAAAA GGGTGATGTT 
    CAGTACTTTA TTGGGGTTCA ATTGGATGGA ACTGAGCATG TTCAGGATGA 
    TGCTGCAAAA GAGGGAGTCG TGCTGGTTAA GAAAACCGCA GATAATATCG 
    ATGAGGCTGC AAAGGAACTC CCGGATGCTA ATTTGAGACC GAAAGATCTA 
    TGGGCTAACC ACTCAAAAGT AGTCCTGCCA AATCCACATA TGAAGGATAC 
    TGCATCATGG AGAGCCATCC AAAAGGTTCT CGAGAGTGGT GAAAGTATTG 
    GTTTAAAACA TTTTAGGCCA GTAAAACCAT TAGGATCTGG TGACACTGGA 
    AGTGTACACT TGGTAGAGTT GTTGAATACA GGAGAATACT TTGCCATGAA 
    AGCTATGGAT AAAAGCATCA TGCTTAACCG CAATAAGGTT CACAGAGCTA 
    CTGCTGAACG CCAAATCCTT GATCTATTAG ATCACCCATT CCTTCCAACA 
    TTATATGCAT CATTTCAGAC AAAAACGCAT ATATGCCTCA TTACTGACTA 
    CTGCCCGGGT GGTGAGCTCT TTGTGCTTCT AGATAATCAA CCTCTGAAGG 
    TTCTACATGA AGATGCAGTC AGGTTCTATG CTGCCGAAGT GGTTGTTGCA 
    CTAGAATACC TACATTGCCA AGGTATAATA TACCGAGACT TAAAACCAGA 
    GAATATCTTA CTTCATAGAG ATGGGCACAT CTCCCTTACA GACTTCGATT 
    TGTCTTGTCT AACATCTTGC AGACCGCAGG TCTTTCTTCC CGAAGATGCT 
    GATGAGAAAA AAGGGAGAAA AAACGGGAGT TACCCCATAT TTTTTGCTGA 
    ACCTATGCGA GCATCCAATT CATTTGTTGG CACGGAGGAG TACATTGCGC 
    CTGAGATTAT TACAGGAGCT GGCCATACAA GCGCTGTTGA TTGGTGGGCT 
    CTAGGGATTC TTCTATATGA AATGTTGTAT GGTTATACAC CTTTTAGAGG 
    AAAAACGAGG CTGCCTTCCT CGCCCGGTGC AAGGCCAACT CCGAGGCCAC 
    GCTCGGCAAG TACGGCGGCG CCGCCGGCGA CGCCGCCACG TCCGAGAGCT 
    TGTACGTCAA GGGCTACACC TACTAGAATT CGTCCCAATC ACTGGGTGGC 
    GCCTATGCGG CCGTGCCTAT GACAGTAGTA GGGTCTCGGT CCAATCCGCG 
    TGGCCCACGT GGCGGTGCGG ATTGCATCAA GATTCGTGCG GATATACATG 
    TGTTCCTTTC TGCTGTATAC TCTCGATTCT CTGCTTCCTT CCCCTCTAGC 
    TCTTATATTT TAACAAAAGC TGCGTCTAAA TGTTC

    Gene models with high sequence identity

    1. AT3G45780.2 [+] show detail - phototropin 1
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Protein kinase, catalytic domain 40.57
    ProfileScan PAS 10.232
    ProfileScan PAS 10.618
    ProfileScan PAS-associated, C-terminal 11.837
    ProfileScan PAS-associated, C-terminal 12.528
    HMMSmart PAS 1.2
    HMMSmart PAC motif 2.2e-05
    HMMSmart PAS 2
    HMMSmart PAC motif 1.6e-08
    HMMSmart Serine/threonine-protein kinase domain 3.4e-69
    HMMSmart Tyrosine-protein kinase, catalytic domain 8.4e-06
    HMMPfam Serine/threonine-protein kinase-like domain 3.4e-55
    HMMPfam PAS fold 3.1e-06
    HMMPfam PAS fold-3 9.2e-06
    Gene3D no description 1.7e-36
    Gene3D no description 7.6e-37
    Gene3D no description 1.1e-45
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    superfamily Protein kinase-like domain 1.7e-68
    superfamily PYP-like sensor domain (PAS domain) 4.8e-25
    superfamily PYP-like sensor domain (PAS domain) 5.5e-25
    HMMTigr PAS 1.3e-08
    HMMTigr PAS 2.6e-09
    HMMPanther NONPHOTOTROPIC HYPOCOTYL PROTEIN 1(PHOTOTROPI 2.2e-148
    HMMPanther SERINE/THREONINE PROTEIN KINASE 2.2e-148

    See the detailed result >

    AT3G45780.2

    Model type
    Protein coding
    Short Description
    phototropin 1
    Curator Summary
    Blue-light photoreceptor. Contains a light activated serine-threonine kinase domain and LOV1 and LOV2 repeats. Mutants are defective in blue-light response. Mediates blue light-induced growth enhancements. PHOT1 and PHOT2 mediate blue light-dependent activation of the plasma membrane H+-ATPase in guard cell protoplasts. PHOT1 undergoes blue-light-dependent autophosphorylation. At least eight phosphorylation sites have been identified in PHOT1. Phosphorylation of serine851 in the activation loop of PHOT1 appears to be required for stomatal opening, chloroplast accumulation, leaf flattening, and phototropism, and phosphorylation of serine849 may also contribute to the regulation of these responses. Phosphorylation-dependent binding of 14-3-3 proteins to the Hinge1 region of PHOT1 appears to require serine350 and serine376.
    Computational Description
    phototropin 1 (PHOT1); FUNCTIONS IN: protein binding, protein serine/threonine kinase activity, FMN binding, kinase activity, blue light photoreceptor activity; INVOLVED IN: in 8 processes; LOCATED IN: internal side of plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: PAC motif (InterPro:IPR001610), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), PAS fold (InterPro:IPR013767), PAS (InterPro:IPR000014), PAS-associated, C-terminal (InterPro:IPR000700), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719); BEST Arabidopsis thaliana protein match is: phototropin 2 (TAIR:AT5G58140.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR