K36644

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K36644

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K36644

Determination of introduced cDNA(s)

  • J013124G04 - Rice Full-length cDNAs - Original: J013124G04

    full reading [+] show sequence

    Nucleotide sequence: J013124G04 - full reading 
    GGTCTGGTCT GGGTCTGGTC GGTCGTCTTC CACTCCATCC GATCGACCAA 
    CCAGCCGATG GGTTCTCGCG GCTGCTTCTG CAAGCGACCC GCGGCCATGG 
    GCAATCTTCT TCTTCTTCTT CCCCACCCGG CCAAGGCCAA ATTGAGGCCT 
    CCCACCACGG CGGCGAAGCG CGGTACACTC TTCTTCTGCA GCCTCAGCAC 
    CGATGGTCCG TCGTCGACGA TGACCGTCTC CATCACTGGC GCCACCGGTT 
    TCGTTGGCCG CAGATTGGTC CAAAAGCTTC TCTCCGAAGA TCACAAGGTT 
    TGCGTCTTAA CGCGGTCCGC CAGCAAGGCC ACATCAGTTT TTCCGGCTTC 
    CACATTTCCA GGAATTACAA TAGCCGAGCA GGGGGACTGG GATAAGTGCA 
    TCCAAGGCTC CACTGCTGTT GTTAACTTGG CAGGCATGCC CATAAGCACA 
    AGGTGGTCAC CTGAGATTAA GAAGGAAATC AAGGAAAGCC GAATCAACGT 
    TACATCAAAA GTGGTGAATT ACATAAATAA TGCCAGTGGT GATGCTCGGC 
    CCTCAGTTTT CGTGAGCGCA ACAGCCATTG GCTTCTATGG TACCAGTGAG 
    ATCAGTAGCT TCGATGAGTC AAGTCCGTCA GGCAATGACT ATCTAGCAGA 
    GGTGTGTCGA GAGTGGGAGG CCAGGGCATG CCAAGTAAAC CAAGAGGATG 
    TTAGGCTTGT GCTTCTTCGG ATTGGGGTTG TTCTCGGCAA GGACGGCGGC 
    GCTCTAGCCA AGATGATTCC CCTTTTTATG ATGTTTGCCG GCGGCCCCCT 
    GGGGACGGGA CGGCAATGGT TTTCCTGGAT TCACCAGGAC GACCTGGTGG 
    ACCTCATCTA CGAGTCCCTC AAGAACCCAT CATATAAAGG CGTGATAAAC 
    GGAACCGCAC CCAACCCGGT TCGGCTGTCG GAGATGTGCG AGCGGCTGGG 
    GCGAGTCGTC GGCCGCCCCT CCTGGCTGCC GGTGCCAGAA ATCGCCCTCA 
    AAGCGGTGCT CGGAGAAGGT GCATCAGTGG TCCTGGAAGG GCAGAAGGTT 
    CTTCCTGTGA AGGCCAAGCA ACTGGGCTTC TCCTACCGAT ACCCCTATGT 
    ACAGGATGCA CTCCGGGCCA TTGCCAGGGA CCTATGACTG ATCGACTCAG 
    ATGAATACTC AAATCGATCG ATCCGCCGTG CACGCACAAC AGTAGATATC 
    AAAATTGAAA TATATTATAT ATACCAAAAG CAAACTATTA TTGTTGTTGG 
    CTTGTATTAT CTCTGATTAA ACTAGGCCAT ACGTTTCATT GGATGGCGTA 
    CGTCTTTCCA CCATTTTTAG ACTGAAGCAA ATATATGCAC GTGAATGAAT 
    GAGC

    Gene models with high sequence identity

    1. AT2G21280.1 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
      E-value: 1e-119; Score: 427.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D NAD(P)-binding domain 1.2e-15
    HMMTigr Sugar nucleotide epimerase YfcH, putative 4.9e-150
    superfamily NAD(P)-binding domain 3.3e-42
    HMMPanther Sugar nucleotide epimerase YfcH, putative 6.7e-78
    HMMPfam NAD-dependent epimerase/dehydratase 3.4e-18
    HMMPfam Domain of unknown function DUF1731, C-terminal 1.7e-16

    See the detailed result >

    AT2G21280.1

    Model type
    Protein coding
    Short Description
    NAD(P)-binding Rossmann-fold superfamily protein
    Curator Summary
    A nuclear-encoded, plastid-targeted protein (AtSulA) whose overexpression causes severe yet stochastic plastid (shown in chloroplasts and leucoplasts) division defects. The protein does not appear to interact with either AtFtsZ proteins when studied in a yeast two-hybrid system.
    Computational Description
    SULA; FUNCTIONS IN: coenzyme binding, binding, catalytic activity; INVOLVED IN: chloroplast fission; LOCATED IN: chloroplast, plastid, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sugar nucleotide epimerase YfcH, putative (InterPro:IPR010099), NAD-dependent epimerase/dehydratase (InterPro:IPR001509), Domain of unknown function DUF1731, C-terminal (InterPro:IPR013549), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G33360.2); Has 4854 Blast hits to 4853 proteins in 1452 species: Archae - 38; Bacteria - 3329; Metazoa - 111; Fungi - 30; Plants - 185; Viruses - 0; Other Eukaryotes - 1161 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR